Biomagnetic Separation - - - -
A Powerful Research Tool for Life Science
------------------------------------------------
Biomagnetic separation techniques have attracted a great deal of
interest in a wide range of applications in biosciences. The techniques
have the advantages for quick, simple and flexible handling of either
large or micro liter scale  biological samples without the need for
laborious repeat of pipetting and centrifuging.
BcMag™ Magnetic Beads are uniform, mono-dispersed, spherical, core−shell
superparamagnetic beads which consist of a nanometer-scale superparamagnetic
iron oxide core completely encapsulated by a high purity silica shell. Silica provides an
excellent chromatography separation medium for purification of target molecules
thanks to its superior mechanical and chemical stability.
Applications:
• Affinity  Beads: Can be used to conjugate almost all function groups
• Reversed-Phase Beads: C4, C8, C18
• Ion-Exchange Beads: WAX, SAX, WCX, SCX
• Immunoprecipitation  & Antibody Purification: Protein A, Protein G, Protein A/G, Protein L
• DNA/RNA Conjugation & Purification: Genomic DNA, cDNA, RNA
• Quick Heteroduplex DNA Finder
• Biotin-related: Streptavidin, monoavidin
• Recombinant Protein  Purification
• Endotoxin Removal.
• Protemics; HSA, IgG….Removal
EDTA, IDA
BioMagnetic Beads
Magnetic Separator
Just Launched
Reversed-Phase Magnetic Beads
Monoavidin Magnetic Beads
Quick Endotoxin Removal
Quick DNA Mutation Finder
BcMag™ Reversed-Phase magnetic beads  
are uniform, silica-based superparamagnetic
beads coated with high density hydrophobic
groups on the surface.  The beads are
specifically designed, tested and quality
controlled for quickly purifying, desalting and
concentrating femtomolar to picomolar scale
of peptides or proteins from biological fluids
and other aqueous samples, manually or
automatically without the need for laborious
repeat of pipetting and centrifuging. Figure 1
is a Typical MALDI-TOF MS spectra (1-10 kDa)
of LMW serum fraction processed on 1um
C18 magnetic beads. (Courtesy of Dr.
Radoslav (Rado) Goldman, Georgetown
University Medical Center )
Features and Advantages:
  • Eluted by mild elution (2mM   biotin)
  • High throughput procedure;
    eliminates columns or filters, or
    laborious repeat of pipetting or
    centrifugation (Fig.1)
  • High binding capacity and elute
    bound biotinylated molecules in  mild
    condition
  • Purifies biotinylated products under
    mild elution conditions
  • Exhibits little nonspecific binding
Features and Advantages:
  • Quick, easy and one-step high-
    throughput procedure (Fig.1)
  • Protein/DNA recovery can reach ~95%
  • Broader working pH range (pH4 -10)
  • No sample dilution
  • Scalable - easily adjusts for sample
    size and automation (µl ~Liter)
  • High binding capacity: 10,000 E.U./ml
  • Beads can be reused at least 5 times.
Features and Advantages:
  • Quick, Easy and one-step high-
    throughput procedure; eliminates
    columns or filters, or laborious repeat
    of pipetting or centrifugation (Fig.1)
  • Identify DNA mutations,deletion, and
    insertion  in high efficient, one-step
    procedure
  • Scalable - easily adjusts for sample
    size and automation
  • Reproducible results
Quick Peptide conjugation & Antibody Purification
Quick DNA/RNA Purification
EcoMag™ Quick Peptide Conjugation Kit  is specifically designed for quick and efficient
conjugation of peptides to Long-arm Iodoaetyl-activated magnetic particles for  antibody
purification from serum of various animal species .

Features and Advantages

Peptide Conjugation
:
  • Covalently  and specifically couples with cysteine residues
  • Water-insoluble ligands  can be conjugated in coupling buffer containing final
    concentration of 10-30% acetonitrile, or dimethylformamide (DMF), or
    dimethylsulfoxide (DMSO), or of  6 M Guanidine•HCl.
  • Stable covalent bond with minimal ligand leakage
  • Produces reusable immunoaffinity matrices
  • Low nonspecific binding
  • Immobilize 1-1.5 mg peptide/ml beads
Antibody Purification:
  • 15-20 minutes protocol
  • Quick, easy-to-use, and one-step procedure; eliminates columns or filters, or
    laborious repeat of pipetting or centrifugation,
  • Low nonspecific binding: Purity >~ 90%
  • Suitable for antibody purification from various animal species
  • No pre-filtration of sample High degree of purity: > ~ 90%
  • High biding capacity: >2mg antibody/ml
  • Low cost
DNA Purification

Features and Advantages:
  • Quick, Easy and one-step high-throughput procedure;
    eliminates columns or filters,or   laborious repeat of
    pipetting or  centrifugation
  • No need for toxic phenol/chloroform.
  • No need for ethanol in Wash buffer to avoid possible residual ethanol in Elution
    buffer.
  • Ultra-pure DNA ready for PCR, restriction digestion and other downstream DNA
    applications



RNA Purification

Features and Advantages

  • Quick, Easy and one-step high-throughput procedure; eliminates columns or filters,
    or laborious repeat of pipetting or centrifugation
  • No need for toxic phenol/chloroform
  • Ultra-pure RNA ready for all downstream RNA applications
  • Low cost
Service
Gene Synthesis
  • Price: Call for quotation
  • 100% Accuracy
  • Codon Optimization: Free
  • Cloning into Any Vector: Free
  • Turnaround Time: 15 Days
  • >>>
Recombinant Protein Production & Purification
  • Just send us your DNA template or amino acid
    sequence , we will return you ultrapure protein
    with low cost
  • >>>
Conjugation to Magetic Beads
  • Just send us you samples, we will conjugate
    them to our magnetic beads with low cost
  • >>>
Special Offer
His-tagged Recombinant Protein Purification
EcoMag His-co2+/Ni2+ Magnetic Beads
40% Off   15ml/only $385
Quick IgG Pure Kit
  • Near Neutral pH Elution
  • Purity: >90%
  • High Binding Capacity: ~5mg/ml of beads
  • Low cost: 100 ml/only $1610
  • >>>
Quick Peptide Conjugation  & Antibody Purification





Long-arm Iodoacetyl-activated Magentic Particles
40% off 1,000 ml/only $12,000
Reversed-phase Magnetic Beads
C4,C8,C18,Phenyl,diPheny. ......
40% off 25ml/only $720
Codon-optimized cDNA and Recombinant Protein
>>> Hundreds of thousands of  Recombinant Proteins and
Codon-optimized cDNA Clones Are Available
The number of recombinant proteins used for academic research and therapeutic applications has increased dramatically.
However, successful recombinant protein expression depends on so many factors such as codon preference, RNA secondary
structure, GC content in heterologous expression systems.  More and more experimental results proved that the expression
level was dramatically increased, from two to one hundred times depending on different genes, when compared with pre-
optimization.  Bioclone has developed a unique cDNA (cloned in E.coli expression Vector, Fig.1) and recombinant protein
(Produced in E.coli Yeast).  Bioclone provides ready-to-use and customer-based service for all the cDNA clones
and recombinant proteins.

Those cDNA clones and recombinant proteins include:
Infection disease antigens (Virus, Bacterial, Parasites),  Nuclear Protein,  Cytokines,  Kinase, G-protein, Receptor,
Phosphatase, Protease,  Signal Transduction,  Apoptosis, Stem Cell and Development, Extracellular Matrix,  
Neuroscience, Toxicology, Drug  metabollism, Common Disease, Transcription factors and Cancer.
Copyright   Bioclone Inc  All rights Reserved
Magnetic Beads and Applications
Affinity Magnetic Beads
Amine Magnetic Beads >>>
Aldehyde Magnetic Beads >>>
Carboxy Magnetic Beads  >>>
CDI Magnetic Bead s>>>
DVS Magnetic Beads  >>>
DADPA Magnetic Beads >>>
Epoxy  Magnetic Beads >>>
Hydrazide Magnetic Beads >>>
Hydroxy Magnetic Beads >>>
Iodoacetyl Magnetic Beads >>>
NHS Magnetic Beads >>>
Sulfhydry Magnetic Beads  >>>
Tosyl Magnetic Beads  >>>
Thiol Magnetic Beads  >>>
Silica Magnetic Beads  >>>
IDA Magnetic Beads >>>
Reversed-Phase  Beads
C4 Magnetic Beads  >>>
C8 Magnetic Beads  >>>
C18 Magnetic Beads  >>>
Cyanopropyl Magnetic Beads  >>>
Phenyl Magnetic Beads  >>>
diPhenyl Magnetic Beads  >>>
Ion Exchange Beads
DEAE  Magnetic Beads  >>>
PSA Magnetic Beads  >>>
SAX Magnetic Beads  >>>
WCX Magnetic Beads  >>>
SCX Magnetic Beads  >>>
Hydroxyapatite  Beads  >>>
Antibody Purification & IP
Protein A Magnetic Beads  >>>
Protein G Magnetic Beads  >>>
Protein A/G  Beads  >>>
Protein L Magnetic Beads  >>>
Quick IgG Pure Beads >>>
Antigen Peptide  Beads >>>
Quick IgM Pure Beads >>>
Anti-IgG Beads >>>
  • Quick IgA Pure Bead
Thiophillic Magnetic Beads  >>>
Antibody Immobilization
Protein A Magnetic Beads  >>>
Protein G Magnetic Beads  >>>
Protein A/G  Beads  >>>
Protein L Magnetic Beads  >>>
Epoxy Magnetic Beads  >>>
Aldehyde Magnetic Beads  >>>
Hydrazide Magnetic Beads  >>>
Carboxy Magnetic Beads  >>>
Iodoacetyl Magnetic Beads  >>>
Thiol Magnetic Beads  >>>
Recombinant Protein Purification
Ni+ Charged Beads
Co+ Charged Beads
Maltose
Calmodulin Beads
Peptide Immobilization
Epoxy Magnetic Beads  >>>
Aldehyde Magnetic Beads  >>>
Carboxyl Magnetic Beads  >>>
Amine Magnetic Beads  >>>
Iodoacetyl Magnetic Beads  >>>
Thiol Magnetic Beads  >>>
DNA/RNA Purification
DNA/RNA Immobilization to Magnetic Beads
Endotoxin Removal Magnetic Beads
Small Molecule Immobilization
Magnetic Beads
Carbohydrate Immobilization
Magnetic Beads
Epoxy Magnetic Beads  >>>
Aldehyde Magnetic Beads  >>>
Hydrazide Magnetic Beads  >>>
DVS Magnetic Beads  >>>
Abundant Protein Removal
  • HSA Removal
  • IgG Removal
  • HSA/IgG Removal
  • Abundant-10 Protein
  • Abundant-22 Protein
 
Cell Isolation
  • Virus
  • Bacteria
  • Cell
  • Parasites
EDTA-Magnetic BEads
Miscellaneous
  • NTP/Oligonucleotides
  • Enzyme
  • Antibody
  • Peptide
  • Boronic Acid
  • Disulfide Reductants
IDA-Magnetic Beads
  • For cDNA or Recombinant Protein Search ( Input  
    Gene name, or Protein Name, or Swiss -prot Accession
    Number
Tel: 1-858-909-0079
Fax: 1-844-273-6046
Email:
info@bioclone.us ;  order@bioclone.us