EcoMag™His-Ni+ Magnetic Particles
EcoMag™ His-Ni Magnetic Particles are silica-based superparamagnetic particles charged with high density Nickle (Ni+2) on
the surface.  The particles are specifically designed  for purification of His-tagged recombinant proteins from different sources.
Compared with Cobalt charged magnetic beads,  the Nickle charged magnetic beads exhibit very high binding capacity  in His-
tagged protein purification especially when His-tagged recombinant protein was expressed at very higher levels.  Therefore,
Cobalt charged magnetic beads are especially suited for purification of His-tagged protein with low level expression.

Features and Advantages:
  • Quick, Easy and one-step high-throughput procedure; eliminates
          columns or filters, or laborious repeat of pipetting or centrifugation (Fig.1)
  • High binding capacity
  • Can be used under denaturing condition (6M Guanadine or 8M Urea)
  • Scalable - easily adjusts for sample size and automation
  • Reproducible  results
  • Lower cost
Need Protein or cDNA?
Hundreds of thousands of  Recombinant
Proteins and Codon-optimized cDNAs
Available
  • Viral Antigen
  • Bacterial Antigen  
  • Kinase  
  • Bacterial Toxin        
  • Parasite Antigen
  • Cancer-related Protein
  • Transcription Factor
  • Human Phosphatase
  • Stem Cell and Development  
  • Signal Transduction
  • CD Marker
  • Drug  metabolism  
  • Neuroscience  
  • Common Disease
  • Cytokine
  • Allergy Protein
  • much more.........   Read More >>>
Bio-Magnetic Purification
A Powerful Tool
for Life Science Research
  • gDNA, cDNA, PCR, Plasmid DNA,
    RNA,
  • Antibody, Peptidde,protein
  • His-,GST-fusion protein
  • Endotoxin  removal
  • Streptavidin, monoavidin
  • Affinity Magnetic Beads -quick  
    conjugation of any molecules
  • Ion Exchange Beads-WAX, SAX, WCX,
    SCX
  • Reversed-Phase Beads: C4, C8, C18
  • Read More >>>>
Gene Synthesis
  • Price: Call for quotation
  • 100% Accuracy
  • Codon Optimization: Free
  • Cloning into Any Vector: Free
  • Turnaround Time: 15 Days
  • Read More >>>
Recombinant Protein Production &
Purification
  • Just send us your  DNA template
    or Expression-ready Clone, we will return
    your highly-purified protein with low cost
Conjugation to Magnetic Beads
  • Send us your samples, we will conjugate
    them to our magnetic beads with low cost.
  • Read More >>>
New Products >>More
How to Order

Email: info@bioclone.us ;  order@bioclone.us
Online: GO >>>
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Magnetic Separator
Order Information
Cat#
Description
Pkg. Size
Price
MHN-104
EcoMag™ Rapid His-tagged Fusion Protein Purification Kit (His-Ni)
Kit components:
2 ml:   EcoMag™ His-Ni magnetic particles
12 ml:   10x Binding/Washing Buffer
2 ml:   2x  Elution Buffer
 
$169
Order>>
MHN-105
EcoMag™ Rapid His-tagged Fusion Protein Purification Kit (His-Ni)
Kit components:
5 ml:   EcoMag™ His-Ni magnetic particles
30ml:   10x Binding/Washing Buffer
5 ml:   2x  Elution Buffer
 
$289
Order>>
MHN-106
EcoMag™ His-Ni magnetic particles
25ml (50mg/ml)
$425
Order>>
MHN-107
EcoMag™ His-Ni magnetic particles
100ml (50mg/ml)
$1490
Order>>
MHN-108
EcoMag™ His-Ni magnetic particles
500ml (50mg/ml)
$5990
Order>>
Large volume  available, Please call for quotation
Product specificities
Composition
Silica-coated iron oxide magnetic beads  charged with Ni+ on the surface
Bead Size
1µm-10µm diameter
Number of Beads
~1.7 x 107 beads/mg
Surface Area
~100 m2/g
Magnetization
~40 EMU/g
Type of Magnetization
Superparamagnetic
Effective Density
2.5 g/ml
Concentration
50 mg/ml (1% NiSO4.6H2O, 20% Ethanol)   
Functional Group Density
>6mg His-tagged GFP /ml of beads
Storage
Store at 4°C upon receipt, Do not freeze
Magnetic Beads and Applications
Affinity Magnetic Beads
  • Amine-terminated
  • Aldehyde-terminated
  • Carboxyl-terminated
  • CDI-activated
  • DVS-activated
  • DADPA-terminated
  • Epoxy-activated
  • Hydrazide-terminated
  • Hydroxyl-terminated
  • Iodoacetyl-activated
  • NHS-activated
  • Sulfhydryl-terminated
  • Tosyl-activated
  • Thiol-activated
  • Silica
 
Reversed-Phase  Beads
  • C4
  • C8
  • C18
  • Cyanopropyl
  • Phenyl
  • diPhenyl
Ion Exchange Beads
  • DEAE (WAX)
  • PSA (WAX)
  • SAX
  • WCX
  • SCX
  • Hydroxyapatite
Antibody Purification & IP
  • Protein A
  • Protein G
  • Protein A/G
  • Protein L
  • Quick IgG Pure Beads
  • Antigen Peptide
  • Quick IgM Pure Beads
  • Anti-IgG Beads
  • Quick IgA Pure Bead
  • Thiophillic Beads
Antibody Immobilization
  • Protein A
  • Protein G
  • Protein A/G
  • Protein L
  • Epoxy-activated
  • Aldehyde-terminated
  • Hydrazide-terminated
  • Carboxyl-terminated
  • Iodoacetyl-activated
  • Thiol-activated
Recombinant Protein
Purification
  • Ni+ Charged Beads
  • Co+ Charged Beads
  • Maltose
  • Calmodulin Beads
Peptide Immobilization
  • Epoxy-activated
  • Aldehyde-terminated
  • Carboxyl-terminated
  • Amine-terminated
  • Iodoacetyl-activated
  • Thiol-activated
DNA/RNA Purification
  • Blood
  • Saliva
  • Stool
  • Food
  • Soil
  • PCR Products
  • mRNA
  • Heteroduplex DNA
    (Mutation Isolation)
DNA/RNA Immobilization
  • Genomic DNA/RNA
  • mRNA
Endotoxin Removal
Small Molecule
Immobilization
Carbohydrate
Immobilization
  • Epoxy-activated
  • Aldehyde-terminated
  • Hydrazide-terminated
  • DVS-activated
Abundant Protein Removal
  • HSA Removal
  • IgG Removal
  • HSA/IgG Removal
  • Abundant-10 Protein
  • Abundant-22 Protein
 
Cell Isolation
  • Virus
  • Bacteria
  • Cell
  • Parasites
EDTA-Magnetic BEads
Miscellaneous
  • NTP
  • Enzyme
  • Antibody
  • Peptide
  • Boronic Acid
  • Disulfide Reductants
IDA-Magnetic Beads
DNA/RNA Purification
>>More
Recombinant Protein
Purification >>More
Antibody
Purification >>More
Small Molecules
Immobilization >>More
Peptide Immobilization
>>More
Carbohydrates
Immobilization >>More
Bacterial,viral,cell
Isolation >>More
MSDS>>
MANUAL>>
Copyright  Bioclone Inc  All rights Reserved
Recombinant Protein Purification
BcMag™ Calmodulin Magnetic Beads
  • Read More>>>
BcMag™ Maltose  Magnetic
Beads
  • Read More>>>
EcoMag™ Co+ charged  Magnetic
Beads
EcoMag™ Ni+ charged  Magnetic
Beads
BcMag™ Co+ charged  Magnetic Bead
BcMag™ Ni+ charged  
Magnetic Beads
BcMag™ Streptavidin Magnetic
Beads

BcMag™ Modoavidin Magnetic
Beads
BcMag™ Glutathione Magnetic Beads
BcMag™ Quick Endotoxin
Removal
Magnetic Beads
 
 
Related Products
  • For cDNA or Recombinant Protein Search ( Input Cat#,
    or Gene name, or Protein Name, or Accession Number
Tel: 1-858-909-0079
Fax: 1-858-909-0057
Email:
info@bioclone.us ;  order@bioclone.us