Reversed-Phase Magnetic Beads
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Monoavidin Magnetic Beads
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Quick Endotoxin Removal
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Quick DNA Mutation Finder
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BcMag™ Reversed-Phase magnetic beads
are uniform, silica-based superparamagnetic
beads coated with high density hydrophobic
groups on the surface. The beads are
specifically designed, tested and quality
controlled for quickly purifying, desalting and
concentrating femtomolar to picomolar scale
of peptides or proteins from biological fluids
and other aqueous samples, manually or
automatically without the need for laborious
repeat of pipetting and centrifuging. Figure 1
is a Typical MALDI-TOF MS spectra (1-10 kDa)
of LMW serum fraction processed on 1um
C18 magnetic beads. (Courtesy of Dr.
Radoslav (Rado) Goldman, Georgetown
University Medical Center )
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Features and Advantages:
- Eluted by mild elution (2mM biotin)
- High throughput procedure;
eliminates columns or filters, or
laborious repeat of pipetting or
centrifugation (Fig.1)
- High binding capacity and elute
bound biotinylated molecules in mild
condition
- Purifies biotinylated products under
mild elution conditions
- Exhibits little nonspecific binding
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Features and Advantages:
- Quick, easy and one-step high-
throughput procedure (Fig.1)
- Protein/DNA recovery can reach ~95%
- Broader working pH range (pH4 -10)
- No sample dilution
- Scalable - easily adjusts for sample
size and automation (µl ~Liter)
- High binding capacity: 10,000 E.U./ml
- Beads can be reused at least 5 times.
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Features and Advantages:
- Quick, Easy and one-step high-
throughput procedure; eliminates
columns or filters, or laborious repeat
of pipetting or centrifugation (Fig.1)
- Identify DNA mutations,deletion, and
insertion in high efficient, one-step
procedure
- Scalable - easily adjusts for sample
size and automation
- Reproducible results
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Quick Peptide conjugation & Antibody Purification
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Quick DNA/RNA Purification
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EcoMag™ Quick Peptide Conjugation Kit is specifically designed for quick and efficient
conjugation of peptides to Long-arm Iodoaetyl-activated magnetic particles for antibody
purification from serum of various animal species .
Features and Advantages
Peptide Conjugation:
- Covalently and specifically couples with cysteine residues
- Water-insoluble ligands can be conjugated in coupling buffer containing final
concentration of 10-30% acetonitrile, or dimethylformamide (DMF), or
dimethylsulfoxide (DMSO), or of 6 M Guanidine•HCl.
- Stable covalent bond with minimal ligand leakage
- Produces reusable immunoaffinity matrices
- Low nonspecific binding
- Immobilize 1-1.5 mg peptide/ml beads
Antibody Purification:
- 15-20 minutes protocol
- Quick, easy-to-use, and one-step procedure; eliminates columns or filters, or
laborious repeat of pipetting or centrifugation,
- Low nonspecific binding: Purity >~ 90%
- Suitable for antibody purification from various animal species
- No pre-filtration of sample High degree of purity: > ~ 90%
- High biding capacity: >2mg antibody/ml
- Low cost
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DNA Purification
Features and Advantages:
- Quick, Easy and one-step high-throughput procedure;
eliminates columns or filters,or laborious repeat of
pipetting or centrifugation
- No need for toxic phenol/chloroform.
- No need for ethanol in Wash buffer to avoid possible residual ethanol in Elution
buffer.
- Ultra-pure DNA ready for PCR, restriction digestion and other downstream DNA
applications
RNA Purification
Features and Advantages
- Quick, Easy and one-step high-throughput procedure; eliminates columns or filters,
or laborious repeat of pipetting or centrifugation
- No need for toxic phenol/chloroform
- Ultra-pure RNA ready for all downstream RNA applications
- Low cost
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