| RattuRat Siah1 (aa 1-282) |
| Siah1 Protein names |
| E3 ubiquitin-protein ligase SIAH1 (EC 6.3.2.-) (Seven in absentia homolog 1) |
| Siah1 Gene names |
| Siah1 (Siah1a) |
| Organism |
| Rattus norvegicus (Rat) |
| Siah1 Entry name |
| SIAH1_RAT |
| Siah1 Codon optimized cDNA Clone |
| cDNA Tag |
| cDNA Siah1 Contains A 6x histidine tag |
| Condon optimized Siah1 CDNA Formulation |
| Liquid |
| Condon optimized Siah1 CDNA Storage |
| Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw |
| Condon optimized Siah1 cDNA Applications |
| Applications: 1. Codon-optimized cDNA sequence designed for recombinant |
| protein production in both E.coli and Mammalian cell. The cDNA has been |
| cloned into E.coli expression vector with 6x Histidine tag.2. May be used as |
| RNAi assistance to restore the loss-of-function. |
Recombinant Siah1 Protein |
| Recombinant Siah1 Protein Cat# |
| PM0573 |
| Siah1 Protein Source |
| Recombinant protein. |
| Recombinant Siah1 Protein Applications |
| Western Blot, may be used for other applications determined by user |
| Recombinant Siah1 Protein Purity |
| >90%, as determined by SDS-PAGE under reducing conditions |
| Recombinant Siah1 Protein Activity |
| N/A |
| Recombinant protein Tag |
| Recombinant Siah1 contains A 6x histidine tag |
| Recombinant Siah1 Protein Formulation |
| Liquid |
| Recombinant Siah1 Protein Storage |
| Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw |
| Siah1 Protein family |
| SINA (Seven in absentia) family |
| Siah1 Subcellular locations |
| Cytoplasm. Nucleus. |
| Siah1 FUNCTION (According to UniProtKB/Swiss Prot) |
| FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and |
| subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases |
| accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a |
| thioester and then directly transfers the ubiquitin to targeted substrates. |
| Mediates E3 ubiquitin ligase activity either through direct binding to |
| substrates or by functioning as the essential RING domain subunit of larger |
| E3 complexes. Triggers the ubiquitin-mediated degradation of many |
| substrates, including proteins involved in transcription regulation (MYB, |
| POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic |
| signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic |
| protein (BAG1), a microtubule motor protein (KIF22), a protein involved in |
| synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) |
| and SNCAIP. It is thereby involved in many cellular processes such as |
| apoptosis, tumor suppression, cell cycle, axon guidance, transcription, |
| spermatogenesis and TNF-alpha signaling. Has some overlapping function |
| with SIAH2. Induces apoptosis in cooperation with PEG3 (By similarity). |
| Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts |
| with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the |
| nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of |
| Siah1 3D structure databases |
| ModBase |
| Siah1 Protein-protein interaction databases |
| STRING |
| Siah1 Proteomic databases |
| PRIDE |
| Siah1 Organism specific databases |
| CTD |
MGI |
| FOR in vitro RESEARCH USE ONLY |
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