Need Protein or cDNA?
Hundreds of thousands of  Recombinant
Proteins and Codon-optimized cDNAs
Available
  • Viral Antigen
  • Bacterial Antigen  
  • Kinase  
  • Bacterial Toxin        
  • Parasite Antigen
  • Cancer-related Protein
  • Transcription Factor
  • Human Phosphatase
  • Stem Cell and Development  
  • Signal Transduction
  • CD Marker
  • Drug  metabolism  
  • Neuroscience  
  • Common Disease
  • Cytokine
  • Allergy Protein
  • much more.........   Read More >>>
Bio-Magnetic Purification
A Powerful Tool
for Life Science Research
  • gDNA, cDNA, PCR, Plasmid DNA,
    RNA,
  • Antibody, Peptidde,protein
  • His-,GST-fusion protein
  • Endotoxin  removal
  • Streptavidin, monoavidin
  • Affinity Magnetic Beads -quick  
    conjugation of any molecules
  • Ion Exchange Beads-WAX, SAX, WCX,
    SCX
  • Reversed-Phase Beads: C4, C8, C18
  • Read More >>>>
Gene Synthesis
  • Price: Call for quotation
  • 100% Accuracy
  • Codon Optimization: Free
  • Cloning into Any Vector: Free
  • Turnaround Time: 15 Days
  • Read More >>>
Recombinant Protein Production &
Purification
  • Just send us your  DNA template
    or Expression-ready Clone, we will return
    your highly-purified protein with low cost
Conjugation to Magnetic Beads
  • Send us your samples, we will conjugate
    them to our magnetic beads with low cost.
  • Read More >>>
New Products >>More
How to Order

Email: info@bioclone.us ;  order@bioclone.us
Online: GO >>>
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Magnetic Separator
Magnetic Beads and Applications
Affinity Magnetic Beads
  • Amine-terminated
  • Aldehyde-terminated
  • Carboxyl-terminated
  • CDI-activated
  • DVS-activated
  • DADPA-terminated
  • Epoxy-activated
  • Hydrazide-terminated
  • Hydroxyl-terminated
  • Iodoacetyl-activated
  • NHS-activated
  • Sulfhydryl-terminated
  • Tosyl-activated
  • Thiol-activated
  • Silica
 
Reversed-Phase  Beads
  • C4
  • C8
  • C18
  • Cyanopropyl
  • Phenyl
  • diPhenyl
Ion Exchange Beads
  • DEAE (WAX)
  • PSA (WAX)
  • SAX
  • WCX
  • SCX
  • Hydroxyapatite
Antibody Purification & IP
  • Protein A
  • Protein G
  • Protein A/G
  • Protein L
  • Quick IgG Pure Beads
  • Antigen Peptide
  • Quick IgM Pure Beads
  • Anti-IgG Beads
  • Quick IgA Pure Bead
  • Thiophillic Beads
Antibody Immobilization
  • Protein A
  • Protein G
  • Protein A/G
  • Protein L
  • Epoxy-activated
  • Aldehyde-terminated
  • Hydrazide-terminated
  • Carboxyl-terminated
  • Iodoacetyl-activated
  • Thiol-activated
Recombinant Protein
Purification
  • Ni+ Charged Beads
  • Co+ Charged Beads
  • Maltose
  • Calmodulin Beads
Peptide Immobilization
  • Epoxy-activated
  • Aldehyde-terminated
  • Carboxyl-terminated
  • Amine-terminated
  • Iodoacetyl-activated
  • Thiol-activated
DNA/RNA Purification
  • Blood
  • Saliva
  • Stool
  • Food
  • Soil
  • PCR Products
  • mRNA
  • Heteroduplex DNA
    (Mutation Isolation)
DNA/RNA Immobilization
  • Genomic DNA/RNA
  • mRNA
Endotoxin Removal
Small Molecule
Immobilization
Carbohydrate
Immobilization
  • Epoxy-activated
  • Aldehyde-terminated
  • Hydrazide-terminated
  • DVS-activated
Abundant Protein Removal
  • HSA Removal
  • IgG Removal
  • HSA/IgG Removal
  • Abundant-10 Protein
  • Abundant-22 Protein
 
Cell Isolation
  • Virus
  • Bacteria
  • Cell
  • Parasites
EDTA-Magnetic BEads
Miscellaneous
  • NTP/Oligonucleotides
  • Enzyme
  • Antibody
  • Peptide
  • Boronic Acid
  • Disulfide Reductants
IDA-Magnetic Beads
Copyright  Bioclone Inc  All rights Reserved
DNA/RNA Purification
>>More
Recombinant Protein
Purification >>More
Antibody
Purification >>More
Small Molecules
Immobilization >>More
Peptide Immobilization
>>More
Carbohydrates
Immobilization >>More
Bacterial,viral,cell
Isolation >>More
Home
BioMagnetic Beads
Recombinant Protein & Codon Optimized cDNA
Service
Order
Contact Us
New Products
Service
Gene Synthesis (Cat# GS-101)

  • FAST TURNAROUNDTIME: 10-15 Business days
  • OPTIMIZE CODON USAGE: Free
  • QUALITY: 100% match with your requested sequences
  • FLEXIBILITY: Free to clone the synthesized gene into any of your desired vector
  • PRODUCTS: Final products (2 μg plasmid or glycerol stock  with detail data sheet )will be delivered.
  • LOWER PRICE: Call or email for quotation
  • To place order, please download ">>>Gene synthesis order form" and Email to order@bioclone.us
Recombinant Protein Production and Purification
E.coli Expression System (Cat# RES-101)
Phase I: Expression vector preparation
1. If customer provides cDNA
  • Subcloning your interest cDNA into bacterial expression vector with His-tag or GST-tag or customer’s expressin  vector.
  • Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperature (16˚ C,
28˚  and 37˚C) by SDS-PAGE or Western blot to check protein solubility,stability and expression level.
  • Estimated time: 5-10 Business days
  • Price: $1,200
2. If customer does not provide cDNA
  • Bioclone will artificially synthesize your interest cDNA , which will be codon-optimized for maximum expression (Normally,
    expression level > 2-100 times higher than original cDNA). clone into expression vector.
  • Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperature (16˚ C,28˚
    and 37˚C) by SDS-PAGE or Western blot to check protein solubility,stability and expression level.
  • Estimated time: 5-10 Business days
  • Price: $1.45 /bp (if cDNA <500bp, $600 will be charge for set-up fee)
Phase II:  Large-scale culture and purification
  • Affinity purification from:  0.5 liter fermented bacterial culture/$590   (Purity>90%)   
  • Affinity purification from:  1.0 liter fermented bacterial culture/$890   (Purity>90%)
  • Estimated time: 5 business days
 Customer will get 2µg plasmid and recombinant protein and detail datasheet
Plant Expression System (Cat# RPS-101)
1. Send us your interest cDNA or protein sequence, we will send you purified His-tagged recombinant protein.
sf9 Insect Cells Expression System (Cat# RIS-101)
Phase I: Expression vector preparation
1. If customer provides cDNA
  • Subcloning your interest cDNA into sf9 expression vector with His-tag and construct bacmid
  • Price $2500
2. If customer does not provide cDNA
  • Bioclon  will optimize codons (codon optimization will dramatically increase expression level as much as > 2-100 times),
    artificially synthesize cDNA, sequencing and cloning into sf9 expression vector with His-tag and construct bacmid.
  • Estimated time: 2-3 weeks
  • Price: $1000+$1.45/bp (if cDNA <1000bp, $600 will be charged for set-up fee)
Phase II: Mini analysis
  • Transfection of sf9 insect cells with bacmid and Plaque purification and viral titer assay.
  •  Virus stock preparation
  • Affinity protein purification from 500ml of culture (Purity >90% determined by SDS-PAGE)
  • Estimated time: 4-6 weeks
  • Price: $3500
 Customer will get 2µg plasmid and Bacmid , purified recombinant and detail datasheet
Transient Mammalian Expression in CHO-K1 or HEK293 (Cat# RMS-101)
Phase I:
1.If customer provides cDNA
  • Cloning of your interest gene into mammalian expression vector with His-tag
  • Mini-scale (5ml) transfection to test the expression level and optimization.
  • Estimated time: 3-4 weeks
  • Price $2,500
2. If customer does not provide cDNA
  • Bioclon will optimize codons (codon optimization will dramatically increase expression level as much as > 2-100 times), artificially
    synthesize cDNA, sequencing and cloning into mammalian expression vector with His-tag.
  • Mini-scale (5ml) transfection to test the expression level and optimization.
  • Estimated time: 4-6 weeks
  • Price: $1500+$1.45/bp (if cDNA <1000bp, $600 will be charge for set-up fee)
Phase II:
Conjugation to Magnetic Beads (Cat# CMS-101)
  • Just send us your protein, or peptide, or DNA or Oligo-nucleotides or Antibody, we will conjugate them to our magnetic Beads.
  • Price: 30 mg of magnetic beads/$990
  • Turnaround time: 1 week
Other Molecular Biology Service
  • For other molecular biology service, please call for quotation !
Home
BioMagnetic Beads
Recombinant Protein & Codon Optimized cDNA
Service
Order
Contact Us
New Products
  • For cDNA or Recombinant Protein Search ( Input Cat#,
    or Gene name, or Protein Name, or Accession Number
Tel: 1-858-909-0079
Fax: 1-858-909-0057
Email:
info@bioclone.us ;  order@bioclone.us