Mouse V(D)J recombination-activating protein 2 (aa 1-527)
V(D)J recombination-activating protein 2 Swiss Prot#
P21784
V(D)J recombination-activating protein 2 Protein names
V(D)J recombination-activating protein 2 (RAG-2)
V(D)J recombination-activating protein 2 Gene names
Rag2 (Rag-2)
Organism
Mouse
V(D)J recombination-activating protein 2 Entry name
RAG2_MOUSE
V(D)J recombination-activating protein 2 Codon optimized cDNA Clone
V(D)J recombination-activating protein 2 CDNA Cat#
RPW0275
cDNA Tag
cDNA V(D)J recombination-activating protein 2 Contains A 6x histidine tag
Condon optimized V(D)J recombination-activating protein 2 CDNA Formulation
Liquid
Condon optimized V(D)J recombination-activating protein 2 cDNA Storage
Ship at Room temperature.. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
Condon optimized V(D)J recombination-activating protein 2 cDNA Applications
Applications: 1. Codon-optimized cDNA sequence designed for recombinant protein production in both E.coli and Mammalian cell. The cDNA has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to
restore the loss-of-function.
Recombinant V(D)J recombination-activating protein 2 Protein
Recombinant V(D)J recombination-activating protein 2 Protein Cat#
PW0275
V(D)J recombination-activating protein 2 Protein Source
Recombinant protein.
Recombinant V(D)J recombination-activating protein 2 Protein Applications
Western Blot, may be used for other applications determined by user
Recombinant V(D)J recombination-activating protein 2 Protein Purity
>90%, as determined by SDS-PAGE under reducing conditions
Recombinant V(D)J recombination-activating protein 2 Protein Activity
N/A
Recombinant protein Tag
Recombinant V(D)J recombination-activating protein 2 Contains A 6x histidine tag
Recombinant V(D)J recombination-activating protein 2 Protein Formulation
Liquid
Recombinant V(D)J recombination-activating protein 2 Protein Storage
Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
Place Order for Recombinant V(D)J recombination-activating protein 2 Protein or cDNA
V(D)J recombination-activating protein 2 Protein family
RAG2 family
V(D)J recombination-activating protein 2 Subcellular locations
Nucleus.
V(D)J recombination-activating protein 2 FUNCTION (According to UniProtKB/Swiss Prot)
FUNCTION: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
V(D)J recombination-activating protein 2 Sequence databases
For cDNA or Recombinant Protein Search ( Input Cat#, or Gene name, or Protein Name, or Accession Number, or the first 60 amino acids sequence from Met)
