SPARC (Secreted protein acidic and rich in cysteine) (Osteonectin) (ON) (Basement-membrane protein 40) (BM-40)
SPARC Gene names
Sparc
Organism
Rat
SPARC Entry name
SPRC_RAT
SPARC Codon optimized cDNA Clone
SPARC CDNA Cat#
RPA0921
cDNA Tag
cDNA SPARC Contains A 6x histidine tag
Condon optimized SPARC CDNA Formulation
Liquid
Condon optimized SPARC cDNA Storage
Ship at Room temperature.. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
Condon optimized SPARC cDNA Applications
Applications: 1. Codon-optimized cDNA sequence designed for recombinant protein production in both E.coli and Mammalian cell. The cDNA has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.
Recombinant SPARC Protein
Recombinant SPARC Protein Cat#
PA0921
SPARC Protein Source
Recombinant protein.
Recombinant SPARC Protein Applications
Western Blot, may be used for other applications determined by user
Recombinant SPARC Protein Purity
>90%, as determined by SDS-PAGE under reducing conditions
Recombinant SPARC Protein Activity
N/A
Recombinant protein Tag
Recombinant SPARC Contains A 6x histidine tag
Recombinant SPARC Protein Formulation
Liquid
Recombinant SPARC Protein Storage
Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
Secreted > extracellular space > extracellular matrix > basement membrane.
SPARC FUNCTION (According to UniProtKB/Swiss Prot)
FUNCTION: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium
binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
For cDNA or Recombinant Protein Search ( Input Cat#, or Gene name, or Protein Name, or Accession Number, or the first 60 amino acids sequence from Met)
