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Human CHEK1 (aa 1-476)
CHEK1 Swiss Prot#
O14757
CHEK1 Protein names
Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)
CHEK1 Gene names
CHEK1 (CHK1)
Organism
Human
CHEK1 Entry name
CHK1_HUMAN
CHEK1 Codon optimized cDNA Clone
CHEK1 CDNA Cat#
RPT0307
cDNA Tag
cDNA CHEK1 Contains A 6x histidine tag
Condon optimized CHEK1 CDNA Formulation
Liquid
Condon optimized CHEK1 CDNA Storage
Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
Condon optimized CHEK1 cDNA Applications
Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant CHEK1 Protein
Recombinant CHEK1 Protein Cat#
PT0307
CHEK1 Protein Source
Recombinant protein.
Recombinant CHEK1 Protein Applications
Western Blot, may be used for other applications determined by user
Recombinant CHEK1 Protein Purity
>90%, as determined by SDS-PAGE under reducing conditions
Recombinant CHEK1 Protein Activity
N/A
Recombinant protein Tag
Recombinant CHEK1 contains A 6x histidine tag
Recombinant CHEK1 Protein Formulation
Liquid
Recombinant CHEK1 Protein Storage
Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
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CHEK1 Protein family
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1

subfamily
CHEK1 Subcellular locations
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
CHEK1 FUNCTION (According to UniProtKB/Swiss Prot)
FUNCTION: Required for checkpoint mediated cell cycle arrest in response

to DNA damage or the presence of unreplicated DNA. May also negatively

regulate cell cycle progression during unperturbed cell cycles. Recognizes

the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates

CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178'

and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding

sites for 14-3-3 proteins which inhibit CDC25A and CDC25C.

Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and

'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity

leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin

complexes and blocks cell cycle progression. Binds to and phosphorylates

RAD51 at 'Thr-309', which may enhance the association of RAD51 with

chromatin and promote DNA repair by homologous recombination. Binds to

and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent

phosphorylation of the chromatin assembly factor ASF1A. This may affect

chromatin assembly during S phase or DNA repair. May also phosphorylate

multiple sites within the C-terminus of TP53, which promotes activation of

TP53 by acetylation and enhances suppression of cellular proliferation.
CHEK1 Sequence databases
EMBL DDBJ GenBank
CHEK1 3D structure databases
ModBase
CHEK1 Protein-protein interaction databases
STRING
CHEK1 Proteomic databases
PRIDE
CHEK1 Genome annotation databases
Ensembl GeneID KEGG UCSC
CHEK1 Organism specific databases
CTD MGI
CHEK1 Phylogenomic databases
eggNOG GeneTree HOGENOM HOVERGEN

InParanoid OrthoDB PhylomeDB OMA
CHEK1 Gene expression databases
Bgee ArrayExpress Genevestigator
CHEK1 Family and domain databases
InterPro PROSITE ProtoNet
FOR in vitro RESEARCH USE ONLY