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Human Mediator of RNA polymerase II transcription subunit 27 (a.a.1-311)

MED27 Swiss Prot#

Q6P2C8

MED27 Protein names

Mediator of RNA polymerase II transcription subunit 27 (Mediator complex

subunit 27) (Cofactor required for Sp1 transcriptional activation subunit 8)

(CRSP complex subunit 8) (Transcriptional coactivator CRSP34) (P37

TRAP/SMCC/PC2 subunit)

MED27 Gene names

MED27 CRSP34 CRSP8

Organism

Human

MED27 Entry name

MED27_HUMAN

MED27 Codon optimized cDNA Clone

MED27 CDNA Cat#

RPS0484

cDNA Tag

cDNA MED27 Contains A 6x histidine tag

Condon optimized MED27 CDNA Formulation

Liquid

Condon optimized MED27 CDNA Storage

Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

Condon optimized MED27 cDNA Applications

Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant MED27 Protein

Recombinant MED27 Protein Cat#

PS0484

MED27 Protein Source

Recombinant protein.

Recombinant MED27 Protein Applications

Western Blot, may be used for other applications determined by user

Recombinant MED27 Protein Purity

>90%, as determined by SDS-PAGE under reducing conditions

Recombinant MED27 Protein Activity

N/A

Recombinant protein Tag

Recombinant MED27 contains A 6x histidine tag

Recombinant MED27 Protein Formulation

Liquid

Recombinant MED27 Protein Storage

Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

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MED27 Protein family

Mediator complex subunit 27 family

MED27 Subcellular locations

Nucleus.

MED27 FUNCTION (According to UniProtKB/Swiss Prot)

FUNCTION: Component of the Mediator complex, a coactivator involved in

the regulated transcription of nearly all RNA polymerase II-dependent genes.

Mediator functions as a bridge to convey information from gene-specific

regulatory proteins to the basal RNA polymerase II transcription machinery.

Mediator is recruited to promoters by direct interactions with regulatory

proteins and serves as a scaffold for the assembly of a functional

preinitiation complex with RNA polymerase II and the general transcription

factors.

MED27 Sequence databases

EMBL

DDBJ

GenBank

MED27 3D structure databases

ModBase

MED27 Protein-protein interaction databases

STRING

MED27 Proteomic databases

PRIDE

MED27 Genome annotation databases

Ensembl

GeneID

KEGG

UCSC

MED27 Organism specific databases

CTD

MGI

MED27 Phylogenomic databases

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

MED27 Gene expression databases

Bgee

ArrayExpress

Genevestigator

MED27 Family and domain databases

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY