| Human E3 ubiquitin-protein ligase SIAH1 (a.a.1-282) |
| SIAH1 Protein names |
| E3 ubiquitin-protein ligase SIAH1 (EC 6.3.2.-) (Seven in absentia homolog 1) |
| SIAH1 Gene names |
| SIAH1 HUMSIAH |
| SIAH1 Entry name |
| SIAH1_HUMAN |
| SIAH1 Codon optimized cDNA Clone |
| cDNA Tag |
| cDNA SIAH1 Contains A 6x histidine tag |
| Condon optimized SIAH1 CDNA Formulation |
| Liquid |
| Condon optimized SIAH1 CDNA Storage |
| Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw |
| Condon optimized SIAH1 cDNA Applications |
| Applications: 1. Codon-optimized cDNA sequence designed for recombinant |
| protein production in both E.coli and Mammalian cell. The cDNA has been |
| cloned into E.coli expression vector with 6x Histidine tag.2. May be used as |
| RNAi assistance to restore the loss-of-function. |
Recombinant SIAH1 Protein |
| Recombinant SIAH1 Protein Cat# |
| PS0760 |
| SIAH1 Protein Source |
| Recombinant protein. |
| Recombinant SIAH1 Protein Applications |
| Western Blot, may be used for other applications determined by user |
| Recombinant SIAH1 Protein Purity |
| >90%, as determined by SDS-PAGE under reducing conditions |
| Recombinant SIAH1 Protein Activity |
| N/A |
| Recombinant protein Tag |
| Recombinant SIAH1 contains A 6x histidine tag |
| Recombinant SIAH1 Protein Formulation |
| Liquid |
| Recombinant SIAH1 Protein Storage |
| Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw |
| SIAH1 Protein family |
| SINA (Seven in absentia) family |
| SIAH1 Subcellular locations |
| Cytoplasm. Nucleus. |
| SIAH1 FUNCTION (According to UniProtKB/Swiss Prot) |
| FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and |
| subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases |
| accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a |
| thioester and then directly transfers the ubiquitin to targeted substrates. |
| Mediates E3 ubiquitin ligase activity either through direct binding to |
| substrates or by functioning as the essential RING domain subunit of larger |
| E3 complexes. Triggers the ubiquitin-mediated degradation of many |
| substrates, including proteins involved in transcription regulation (MYB, |
| POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic |
| signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic |
| protein (BAG1), a microtubule motor protein (KIF22), a protein involved in |
| synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) |
| and SNCAIP. It is thereby involved in many cellular processes such as |
| apoptosis, tumor suppression, cell cycle, axon guidance, transcription |
| regulation, spermatogenesis and TNF-alpha signaling. Has some |
| overlapping function with SIAH2. Induces apoptosis in cooperation with |
| PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, |
| interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH |
| to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the |
| degradation of nuclear proteins. |
| SIAH1 3D structure databases |
| ModBase |
| SIAH1 Protein-protein interaction databases |
| STRING |
| SIAH1 Proteomic databases |
| PRIDE |
| SIAH1 Organism specific databases |
| CTD |
MGI |
| FOR in vitro RESEARCH USE ONLY |
|
|
