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Human Macrophage migration inhibitory factor (a.a.2–115)
MIF Swiss Prot#
P14174
MIF Protein names
Macrophage migration inhibitory factor (MIF) (EC 5.3.2.1) (Phenylpyruvate

tautomerase) (L-dopachrome tautomerase) (EC 5.3.3.12) (L-dopachrome

isomerase) (Glycosylation-inhibiting factor) (GIF)
MIF Gene names
MIF (GLIF) (MMIF)
Organism
Human
MIF Entry name
MIF_HUMAN
MIF Codon optimized cDNA Clone
MIF CDNA Cat#
RPE0411
cDNA Tag
cDNA MIF Contains A 6x histidine tag
Condon optimized MIF CDNA Formulation
Liquid
Condon optimized MIF CDNA Storage
Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
Condon optimized MIF cDNA Applications
Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant MIF Protein
Recombinant MIF Protein Cat#
PE0411
MIF Protein Source
Recombinant protein.
Recombinant MIF Protein Applications
Western Blot, may be used for other applications determined by user
Recombinant MIF Protein Purity
>90%, as determined by SDS-PAGE under reducing conditions
Recombinant MIF Protein Activity
N/A
Recombinant protein Tag
Recombinant MIF contains A 6x histidine tag
Recombinant MIF Protein Formulation
Liquid
Recombinant MIF Protein Storage
Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
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MIF Protein family
MIF family
MIF Subcellular locations
Secreted. Cytoplasm.
MIF FUNCTION (According to UniProtKB/Swiss Prot)
FUNCTION: Pro-inflammatory cytokine. Involved in the innate immune

response to bacterial pathogens. The expression of MIF at sites of

inflammation suggests a role as mediator in regulating the function of

macrophages in host defense. Counteracts the anti-inflammatory activity of

glucocorticoids. Has phenylpyruvate tautomerase and dopachrome

tautomerase activity (in vitro), but the physiological substrate is not known.

It is not clear whether the tautomerase activity has any physiological

relevance, and whether it is important for cytokine activity.
MIF Sequence databases
EMBL DDBJ GenBank
MIF 3D structure databases
ModBase
MIF Protein-protein interaction databases
STRING
MIF Proteomic databases
PRIDE
MIF Genome annotation databases
Ensembl GeneID KEGG UCSC
MIF Organism specific databases
CTD MGI
MIF Phylogenomic databases
eggNOG GeneTree HOGENOM HOVERGEN

InParanoid OrthoDB PhylomeDB OMA
MIF Gene expression databases
Bgee ArrayExpress Genevestigator
MIF Family and domain databases
InterPro PROSITE ProtoNet
FOR in vitro RESEARCH USE ONLY