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Human Mothers against decapentaplegic homolog 3 (a.a.1–425)

SMAD3 Swiss Prot#

P84022

SMAD3 Protein names

Mothers against decapentaplegic homolog 3 (Mad3) (hMAD-3) (Mothers

against DPP homolog 3) (SMAD 3) (hSMAD3) (JV15-2)

SMAD3 Gene names

SMAD3 (MADH3)

Organism

Human

SMAD3 Entry name

Keratin, type I cytoskeletal 20 (Cytokeratin-20) (CK-20) (Keratin-20) (K20) (Protein IT)

SMAD3 Codon optimized cDNA Clone

SMAD3 CDNA Cat#

RPE0702

cDNA Tag

cDNA SMAD3 Contains A 6x histidine tag

Condon optimized SMAD3 CDNA Formulation

Liquid

Condon optimized SMAD3 CDNA Storage

Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

Condon optimized SMAD3 cDNA Applications

Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant SMAD3 Protein

Recombinant SMAD3 Protein Cat#

PE0702

SMAD3 Protein Source

Recombinant protein.

Recombinant SMAD3 Protein Applications

Western Blot, may be used for other applications determined by user

Recombinant SMAD3 Protein Purity

>90%, as determined by SDS-PAGE under reducing conditions

Recombinant SMAD3 Protein Activity

N/A

Recombinant protein Tag

Recombinant SMAD3 contains A 6x histidine tag

Recombinant SMAD3 Protein Formulation

Liquid

Recombinant SMAD3 Protein Storage

Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

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SMAD3 Protein family

Dwarfin/SMAD family

SMAD3 Subcellular locations

Cytoplasm. Nucleus.

SMAD3 FUNCTION (According to UniProtKB/Swiss Prot)

FUNCTION: Receptor-regulated SMAD (R-SMAD) that is an intracellular

signal transducer and transcriptional modulator activated by TGF-beta

(transforming growth factor) and activin type 1 receptor kinases. Binds the

TRE element in the promoter region of many genes that are regulated by TGF-

beta and, on formation of the SMAD3/SMAD4 complex, activates

transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-

1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory

effect on wound healing probably by modulating both growth and migration of

primary keratinocytes and by altering the TGF-mediated chemotaxis of

monocytes. This effect on wound healing appears to be hormone-sensitive.

Regulator of chondrogenesis and osteogenesis and inhibits early healing of

bone fractures (By similarity).

SMAD3 Sequence databases

EMBL

DDBJ

GenBank

SMAD3 3D structure databases

ModBase

SMAD3 Protein-protein interaction databases

STRING

SMAD3 Proteomic databases

PRIDE

SMAD3 Genome annotation databases

Ensembl

GeneID

KEGG

UCSC

SMAD3 Organism specific databases

CTD

MGI

SMAD3 Phylogenomic databases

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

SMAD3 Gene expression databases

Bgee

ArrayExpress

Genevestigator

SMAD3 Family and domain databases

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY