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Human E3 ubiquitin-protein ligase Mdm2 (a.a.1–491)

MDM2 Swiss Prot#

Q00987

MDM2 Protein names

E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (p53-binding protein Mdm2)

(Oncoprotein Mdm2) (Double minute 2 protein) (Hdm2)

MDM2 Gene names

MDM2

Organism

Human

MDM2 Entry name

SRBP1_HUMAN

MDM2 Codon optimized cDNA Clone

MDM2 CDNA Cat#

RPE0773

cDNA Tag

cDNA MDM2 Contains A 6x histidine tag

Condon optimized MDM2 CDNA Formulation

Liquid

Condon optimized MDM2 CDNA Storage

Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

Condon optimized MDM2 cDNA Applications

Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant MDM2 Protein

Recombinant MDM2 Protein Cat#

PE0773

MDM2 Protein Source

Recombinant protein.

Recombinant MDM2 Protein Applications

Western Blot, may be used for other applications determined by user

Recombinant MDM2 Protein Purity

>90%, as determined by SDS-PAGE under reducing conditions

Recombinant MDM2 Protein Activity

N/A

Recombinant protein Tag

Recombinant MDM2 contains A 6x histidine tag

Recombinant MDM2 Protein Formulation

Liquid

Recombinant MDM2 Protein Storage

Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

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MDM2 Protein family

MDM2/MDM4 family

MDM2 Subcellular locations

Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus.

MDM2 FUNCTION (According to UniProtKB/Swiss Prot)

FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination of

p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53-

and p73/TP73-mediated cell cycle arrest and apoptosis by binding its

transcriptional activation domain. Also acts as an ubiquitin ligase E3

toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes

proteasome-dependent ubiquitin-independent degradation of retinoblastoma

RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination

and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex

involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-

ERBB4-MDM2 complex which links growth factor and DNA damage

response pathways.

MDM2 Sequence databases

EMBL

DDBJ

GenBank

MDM2 3D structure databases

ModBase

MDM2 Protein-protein interaction databases

STRING

MDM2 Proteomic databases

PRIDE

MDM2 Genome annotation databases

Ensembl

GeneID

KEGG

UCSC

MDM2 Organism specific databases

CTD

MGI

MDM2 Phylogenomic databases

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

MDM2 Gene expression databases

Bgee

ArrayExpress

Genevestigator

MDM2 Family and domain databases

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY