EcoMag™ Protein L Magnetic Particles
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EcoMag™ Protein L Magnetic Beads are silica-based superparamagnetic beads covalently conjugated with recombinant
protein L (Purity: >80%) on the surface. Unlike protein A, protein G or protein A/G, protein L binds kappa light chains (i.e., k
chain of the VL domain) instead of Fc region (i.e., heavy chain) of immunoglobulins. Therefore, protein L beads not only bind
IgG, but also other classes of Ig including IgM, IgA, IgE and IgD, and single chain variable fragments (ScFv) and Fab
fragments. . The Beads are specifically designed, tested and quality controlled for use in quick and efficient one-step
purification of monoclonal antibodies from ascites or culture supernatants because it does not bind bovine immunoglobulins
Features and Advantages
- Quick, Easy and one-step high-throughput procedure; eliminates columns or filters, or laborious repeat of pipetting or
centrifugation (Fig.1)
- Binds IgG, IgM, IgA, IgE and IgD
- Binds single-chain variable fragments (ScFv)
- Not bind bovine, goat or sheep Igs
- Ultra low non-specific binding thanks to the beads’ hydrophilic surface
- High binding capacity
- Scalable - easily adjusts for sample size and automation
- Low cost
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