Steroids, dyes,drugs and etc are classes of small molecules, which are difficult or impossible to be immobilized by current immobilization methods due to either absence or inaccessibility of easily reactive functional groups. However, those small molecules may be possibly immobilized to DADPA-terminated magnetic beads via their active hydrogens being condensed with formaldehyde and an amine in the Mannich reaction.
Features and Advantages:
High binding capacity
Fast, efficient coupling
Hydrophillic long-arm spacer minimizing
steric hindrance and non-specific binding.
Silica-coated iron oxide magnetic beads grafted with DADPA group on the surface
1µm diameter; ~5µm diameter
Number of Beads
~1.7 x 108 beads/mg; ~5 x 107 beads (5µm beads) /mg
Type of Magnetization
pH 4-10;Temperature: 4°C -140°C; Most organic solvents